Clay-Based Affinity Probes for the Selective Cleanup and Analysis of Aflatoxin B1 Utilizing Nanostructured Montmorillonite on Quartz.

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Henry J. Huebner; Timothy D. Phillips

Type of Document:
Scholarly Article


Journal of the Association of Official Analytical Chemists

Date of Publication:

Place of Publication:
Not Available


Abstract: A study was conducted to investigate the selective cleanup and determination of aflatoxin B1 (AfB1) from contaminated media. Composite adsorbents were formulated from calcium montmorillonite clay, which possesses a high affinity and enthalpy of adsorption for AfB1. Nanostructuring techniques were used to construct various formulations of the clay-based composite media. In AfB1 adsorption studies with prototypical affinity columns, these composites offered narrowly defined, reproducible capacity ranges. Composite recoveries of AfB1 from spiked grains exhibited linear trends that correlated well with the range of spike levels. Composite columns provided lower recoveries of AfB1 from naturally contaminated corn than did immunoaffinity columns; however, recoveries were consistent and purified extracts were free of interfering compounds, as determined by liquid chromatography with fluorescence detection.

Additional Bibliographic Information

Journal of the Association of Official Analytical Chemists International 86:534-539

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