In vitro reproductive development of peanut, Arachis hypogaea L. as influenced by plant growth regulators, sucrose and pH
QL Feng; HE Pattee; HT Stalker
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Abstract: Research on in vitro embryo culture in Arachis has the objectives ofrescuinginterspecific hybridembryos which abort before they reach maturity. This study explored effects ofthe three exogenous plant growth regulators 1- naphthaleneacetic acid (NAA), gibberellic acid (GA,), and 6-benzylaminopurine (6-BAP); sucrose; and medium pH on in vitro fruit and embryo development ofA.
hypogaea L. by culturing 10-d-old peg tips. Results indicated that medium containing 0.5 to 1.0 mg L NAA was optimal for in vitro pod formation and embryo development. GA, did not have a significant influence and 6-BAP had negative effects on both in vitro fruit and embryo development. High concentrations of 6-BAP and NAA induced callus which inhibited ovary enlargement and embryo development. Sixty g L-‘ sucrose was the best concentration for ovary enlargement and embryo development. Acidic medium was needed for in vitro reproductive development with pH 4.5-6.5 the most favorable. A pod formation frequency of 81%, a seed production rate of 90% (from pods recovered in