Molecular Identification of Viruses of Solanaceous and Cucurbit Crops in Honduras and Transgenic Resistance to Control Tomato Mosaic and Pepper Golden Mosaic Viruses in Tomato
EAP, Zamorano (Honduras); University of Arizona, University of Georgia, Purdue University
A. Rueda, M. M. Roca de Doyle (EAP, Zamorano); D.T. Krigsvold, J.M. Rivera, J.C. Melgar (FHIA); R. Martyn (Purdue University); J. Brown (U of Arizona); M. Deom, (U. o f Georgia
This project focused on identifying important plant viruses that limit the production of cucurbits, pepper and tomato in Honduras and developing the diagnostic infrastructure at FHIA’s and Zamorano’s laboratories to conduct ELISA and PCR tests for virus identification and detection.
1. To identify the most important plant viruses that constrain the production of cucurbits, pepper, and tomato in Honduras (and Central America), and (b) develop the diagnostic infrastructure at FHIA and Zamorano to enable the two laboratories to carry out ELISA and PCR for virus identification and detection. 2. Cloning of previously uncharacterized begomoviruses of tomato, molecular characterization, and demonstration of the causal nature. 3. To develop disease resistant tomato plants using virus-derived resistance strategies by targeting three of the most destructive viruses on tomato and pepper in Honduras and Central America: Tobacco etch (TEV) and Tomato mosaic(ToMV) viruses, single-stranded, positive-sense RNA viruses of the genus:Potyvirus and Tobamovirus, respectively, and Pepper golden mosaic virus(PePGMV), a single-stranded DNA virus of the genus: Begomoviruswith a bipartite genome (Brown et al. in preparation; Years 8,9 IPM CRSP Project; Year 10 ToMV was found to be particularly severe and widespread).
1.Established and employed ELISA and PCR methodologies for detection and identification of important plant viruses at FHIA and ZAMORANO, independently. 2.Completed a three-year study in Honduras to identify and archive predominant DNA-and RNA-viruses of tomato, pepper, watermelon, and various oriental vegetables (year 2003-04),which will be targeted in disease resistance objectives. Knowledge of the extent of Begomovirus diversity in Honduran crop species will be available for the first time and can be applied to disease resistance development (final year in progress). 3.Obtained infectious clones and completed Koch's Postulates for two previously uncharacterized begomoviruses of tomato and pepper in the region (in progress). 4.Publish joint scientific abstracts, Disease Notes,and/or articles describing results. Cloned viral CP and/or REP gene sequences from the Honduran ToMV and PepGMV isolates and determined the sequence.