Description

Abstract: Nisin-incorporated biopolymers (methylcellulose (MC), hydroxypropylmethyl cellulose (HPMC), ?-carrageenan and chitosan) films made by heat-press and casting methods were investigated. For quantitative determinations of nisin release in both categories of prepared films, the plate diffusion method was employed using Micrococcus luteus ATCC 10240 as the test microorganism. Cast films exhibited larger inhibitory zones overall when compared with that of the heat-pressed method. Among all the nisin-incorporated films assayed, the antimicrobial activity was most effective in MC films in the heat-pressed category and chitosan films in the cast films category.