Determination of quercetin concentrations in fi sh tissues after feeding quercetin-containing diets

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Kwan Ha Park; Gustavo A. Rodriguez-Montes de Oca; Pierluigi Bonello; Kyeong-Jun Lee; Konrad Dabrowski

Type of Document:
Scholarly Article


Aquaculture International

Date of Publication:

Place of Publication:
Not Available


Abstract: Concentrations of quercetin in fi sh tissues were measured for the fi rst time using HPLCelectrochemical

detection method. Its identity was also ascertained with UV-photodiode array detection. Quercetin, in aglycone form, was at measurable concentrations in tilapia plasma, liver, and whole body homogenate when fed with diets containing 1% quercetin (aglycone) for 1 or 15 weeks. Hydrolysis with glucuronidase/sulfatase treatment for the purpose of cleaving conjugates did not increase quercetin levels, suggesting that glucoronide or sulfate conjugates are not the major metabolic forms in Nile tilapia (Oreochromis niloticus). No quercetin was detected in plasma of rainbow trout (Salmo gairdneri) or white sturgeon (Acipenser transmontanus) fed commercial diets. The results suggest that quercetin is absorbed in tilapia and that this fl avonoid is deposited mainly in aglycone form in the body after absorption.

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