Developing Improved Management Strategies for Disease Caused by Phytophthora Infestans in Solanaceous Crops Using Molecular Biological Tools
INIAP-EE Santa Catalina; CIP-Lima; CIP-Quito
Gustavo Bernal, Jose Ochoa, Veronica Galarza (INIAP-E.E. Santa Catalina); Greg Forbes (CIP-Lima); Francisco Jarrin (CIP-Quito) (Ecuador)
This research activity focused on developing improved management strategies in Ecuador for coping with Phytophthora infestans, a pathogen with notoriety for the destructive disease it cauess in both Naranjill and tree tomato.
1. Equip the laboratory of the Plant Protection Department in the National Institute of Agricultural research (INIAP) for this type of work. 2. Train a research assistant from INIAP for one year in International Potato Center (CIP) to use molecular markers. 3. Collect representative isolates of P. infestans in the major production areas of tree tomato and naranjilla cultivation in Ecuador. 4. Adjust protocols for isolation and pathogenicity studies of P. infestans in tree tomato and naranjilla. 5. Identify the mating type of P. infestans isolates of naranjilla and tree tomato 6. Evaluate the levels of metalaxyl sensitivity of P.infestans isolates of naranjilla and tree tomato. 7. Identify P. infestans hyplotypes in the tree tomato and naranjilla fungus population.
Researchers from the Department of the Plant Protection department of Santa Catalina Experimental Station of INIAP are being trained in conversional and molecular techniques of late blight. Information of mating types and metalaxyl sensitivity is available for a representative population of tree tomato and naranjilla late blight. Inoculation methods of P. infestans for tree tomato and naranjilla are being adjusted.