High pressure liquid chromatographic determination of zearaleonone in chicken tissues

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GW Turner; TD Phillips; ND Heidelbaugh; LH Russel

Type of Document:
Scholarly Article


Journal of the Association of Official Analytical Chemists

Date of Publication:

Place of Publication:
Not Available


Abstract: A method is reported for the extraction and analysis of zearalenone in chicken fat, heart muscle, and kidney tissue by using high pressure liquid chromatography (HPLC). Zearalenone is extracted with acetonitrile, cleaned up with hexane, and extracted further with ethyl acetate. Zearalenone is determined by HPLC using a reverse phase radial compression separation system, an ultraviolet absorbance detector, and a mobile phase of acetonitrile-water (60 + 40) (v/v). Recoveries of zearalenone added at levels from 50 to 200 ng/g are in the range 82.6-95.1%.

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